Protocol: Analysis of FTA cards for Leishmania parasites

Protocol for analysis of FTA cards for Leishmania parasites. Describes the process of gDNA or RNA extraction from FTA cards (from lab or field work), and real time PCR for detection of parasites. Limits of detection for the PCR assay are also provided.

Protocol from the Gnatwork project “The use of FTA cards to monitor Leishmania infection and infectiousness in sand flies and midges“ created for processing of samples collected during laboratory work at LSHTM and field work in Ethiopia and Ghana (Oct 2018 – Sep 2019).