Protocol: Cas9 Targeted Sequencing Protocol for Simulium sp and Onchocerca volvulus

03 Jul 2020
Samuel Armoo, Romain Derelle, Daniel Boakye, Mike Y. Osei-Atweneboana, Charles Brockhouse, John Colbourne.

This protocol is for the preparation of Cas9 guide RNA targeted MinION sequencing library from DNA extracted from Simulium sp and Onchocerca volvulus worms. This protocol also describes DNA library loading procedures. This protocol is adapted from Gilpatrick et al. (2020)

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GN_23 Preparation of Cas9 guide RNA targeted MinION sequencing library
nanopore sequencing