Protocol: Detection of Onchocerca spp infection in Simulium damnosum s.l. by loop-mediated isothermal amplification (LAMP).

15 Jan 2021
Ya’cob, Z. Poole, C. Bah, GS. Carlow, T. Makepeace, BL. Graham-Brown, J.

A LAMP assay protocol developed for detecting (a) Onchocerca spp infection in Simulium damnosum s.l. populations using a genus-specific O-150 primer set and (b) Onchocerca volvulus infection in Simulium damnosum s.l. populations using a species-specific OvGST-1α primer set. Both methods are intended for use on pooled samples (n=100) of S. damnosum s.l. flies to determine infection status within the population of interest. 

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GN_27 Detection of Onchocerca spp infection in Simulium damnosum s.l. by loop-mediated isothermal amplification.pdf