06 Sep 2018 Protocol: Non-destructive DNA extraction Non-destructive DNA extraction from dipteran specimens
06 Sep 2018 Protocol: Destructive DNA extraction DNA extraction from Dipteran specimens, whole blood and cultured cells using Qiagen DNeasy spin columns
06 Sep 2018 Protocol: COI barcoding PCR Amplification of the DNA Barcode Segment of the mitochondrial DNA Cytochrome Oxidase Subunit I (COI) gene
06 Sep 2018 Protocol: Agarose gel electophoresis Agarose Gel Electrophoresis using SYBR Safe DNA gel stain
06 Sep 2018 Protocol: Pre-cast E-Gel agarose electrophoresis Agarose Gel Electrophoresis using Pre-Cast High Throughput SYBR Safe E-Gels
06 Sep 2018 Protocol: Good laboratory practice DNA Barcoding introduction to good laboratory practice guide
11 Nov 2019 Protocol: Identification of blood meal source from dipteran specimens Multiplex species-specific PCR-based assays to identify blood meal source
06 Jan 2020 Protocol: Staining small biting flies prior to Micro-CT scanning Protocol for preparing and staining sandflies and blackflies prior to micro-computed tomography scanning
30 Mar 2020 Protocol: Bovine milk casein ELISA protocol This protocol is for the detection of milk casein on small marked arthropods for dispersal and habitat studies.
04 May 2020 Protocol: Use of FTA cards for Leishmania parasite detection in laboratory-infected sand flies and midges
04 May 2020 Protocol: Analysis of FTA cards for Leishmania parasites Nucleic acid extraction and qPCR for detection of parasites from FTA cards
05 Jun 2020 Protocol: Egg-white ovalbumin ELISA protocol This protocol is for the detection of egg-white ovalbumin on small marked arthropods including Phlebotomine sand flies and Culicoides biting midges for dispersal and habitat studies.
03 Jul 2020 Protocol: Cas9 Targeted Sequencing Protocol for Simulium sp and Onchocerca volvulus This protocol is for the preparation of Cas9 guide RNA targeted MinION sequencing library from DNA extracted from Simulium sp and Onchocerca volvulus worms. This protocol also describes DNA library loading procedures. This protocol is adapted from Gilpatrick et al. (2020)
06 Jul 2020 Protocol: DNA Quality Control Check This protocol is for assessing DNA quality and yield prior to sequencing library preparation. This is a very important step given the low affinity of sequencing adapter ligases when it comes to poor DNA quality. Adapted from NanoDrop spectrophotometer (Thermo Scientific), and Qubit fluorometer (ThermoFisher Scientific) manufacturer’s manuals.
15 Jan 2021 Protocol: Detection of Onchocerca spp infection in Simulium damnosum s.l. by loop-mediated isothermal amplification (LAMP).
04 Jan 2021 Protocol: Immunoassays to measure IgG or IgM anti-S. damnosum s.l. antibodies in humans This protocol is for performing two separate immunoassays to measure IgG or IgM anti-S. damnosum s.l. antibodies in humans.
