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July 2022

Protocol: Emergence Trapping

A method for collecting emerging Culicoides
Protocol
07 May 2021

Key to females of economically important species of Culicoides subgenus Avaritia from southern Asia and Australasia using characters visible under a stereomicroscope

Protocol
07 Apr 2021

Morphological identification key for the Culicoides fauna of Ghana

Protocol
27 Jan 2021

Protocol: Host Decoy Trap Protocol

This protocol is for Host Decoy Traps for the outdoor collection of hematophagous insects.
Protocol
15 Jan 2021

Protocol: Detection of Onchocerca spp infection in Simulium damnosum s.l. by loop-mediated isothermal amplification (LAMP).

Protocol
05 Jan 2021

Protocol: Field collection and salivary gland dissection of blackflies

This protocol for field collection and salivary gland dissection of blackflies.
Protocol
04 Jan 2021

Protocol: Immunoassays to measure IgG or IgM anti-S. damnosum s.l. antibodies in humans

This protocol is for performing two separate immunoassays to measure IgG or IgM anti-S. damnosum s.l. antibodies in humans. 
Protocol
06 Jul 2020

Protocol: MinION FASTQ DNA Sequence Analysis

Protocol
06 Jul 2020

Protocol: DNA Quality Control Check

This protocol is for assessing DNA quality and yield prior to sequencing library preparation. This is a very important step given the low affinity of sequencing adapter ligases when it comes to poor DNA quality. Adapted from NanoDrop spectrophotometer (Thermo Scientific), and Qubit fluorometer (ThermoFisher Scientific) manufacturer’s manuals.
Protocol
06 Jul 2020

Protocol: DNA Extraction Protocol for Simulium sp. and Onchocerca volvulus

Protocol
03 Jul 2020

Protocol: Cas9 Targeted Sequencing Protocol for Simulium sp and Onchocerca volvulus

This protocol is for the preparation of Cas9 guide RNA targeted MinION sequencing library from DNA extracted from Simulium sp and Onchocerca volvulus worms. This protocol also describes DNA library loading procedures. This protocol is adapted from Gilpatrick et al. (2020)
Protocol
05 Jun 2020

Protocol: Egg-white ovalbumin ELISA protocol

This protocol is for the detection of egg-white ovalbumin on small marked arthropods including Phlebotomine sand flies and Culicoides biting midges for dispersal and habitat studies.
Protocol

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